Analysis of complex lipid mixtures by thin‐layer chromatography and complementary methods

作者：Donald C. Malins, Helmut K. Mangold · 发表于：Journal of the American Oil Chemists Society · 年份：1960 · DOI：10.1007/bf02631604 · 被引用次数：390 · 研究领域：Analytical Chemistry and Chromatography、Chromatography in Natural Products、Protein purification and stability

Conclusion Further work has been done on the application of thin‐layer adsorption chromatography to the fractionation of complex lipid mixtures into classes. New methods, the use of siliconized silicic acid plates and the application of thin‐layer adsorption chromatography combined with the complementary techniques of gas‐liquid chromatography and paper chromatography, are presented for the resolution of classes of lipids into their constituents. In contrast to such elaborate conventional techniques as column chromatography, analyses using the methods reported in this paper can be performed rapidly in large numbers on a routine basis.