Characterization of histone genes isolated fromXenopus laevisandXenopus tropicalisgenomic libraries

作者：Ida Rubati, Paola Fragapane, Paola Pierandrei‐Amaldi, Elena Beccari, Francesco Amaldi, Irene Bozzoni · 发表于：Nucleic Acids Research · 年份：1982 · DOI：10.1093/nar/10.23.7543 · 被引用次数：42 · 研究领域：Genomics and Phylogenetic Studies、Genomics and Chromatin Dynamics、Insect Resistance and Genetics

Using a cDNA clone for the histone H3 we have isolated, from two genomic libraries of Xenopus laevis and Xenopus tropicalis, clones containing four different histone gene clusters. The structural organization of X. laevis histone genes has been determined by restriction mapping, Southern blot hybridization and translation of the mRNAs which hybridize to the various restriction fragments. The arrangement of the histone genes in X. tropicalis has been determined by Southern analysis using X. laevis genomic fragments, containing individual genes, as probes. Histone genes are clustered in the genome of X. laevis and X. tropicalis and, compared to invertebrates, show a higher organization heterogeneity as demonstrated by structural analysis of the four genomic clones. In fact, the order of the genes within individual clusters is not conserved.