Fate mapping the avian neural plate with quail/chick chimeras: Origin of prospective median wedge cells

作者：Gary C. Schoenwolf, Hilde Elisa Bortier, Lucien C. A. Vakaet · 发表于：Journal of Experimental Zoology · 年份：1989 · DOI：10.1002/jez.1402490305 · 被引用次数：69 · 研究领域：Congenital heart defects research、Neurogenesis and neuroplasticity mechanisms、Developmental Biology and Gene Regulation

The origin of prospective M cells, which are median neuroepithelial cells that become wedge-shaped during bending of the neural plate and eventually form the midline floor of the neural tube, was determined by constructing quail/chick chimeras and using the quail nucleolar marker to identify quail donor cells in chick host blastoderms. Two possible sites of prospective M-cell origin in the epiblast were examined: a single, midline rudiment located just rostral to Hensen's node and paired rudiments flanking the cranial part of the primitive streak. Our results suggest that M cells arise exclusively from the midline, prenodal rudiment. From this rudiment, M cells extend caudally throughout the entire length of the neuroepithelium. This new information on the origin of prospective M cells will aid in the analysis of their role in neurulation.