Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria

作者：Stephan Heeb, Yoshifumi Itoh, Takayuki Nishijyo, Ursula Schnider, Christoph Keel, Julie Wade, U. F. Walsh, Fergal O’Gara, Dieter Haas · 发表于：Molecular Plant-Microbe Interactions · 年份：2000 · DOI：10.1094/mpmi.2000.13.2.232 · 被引用次数：380 · 研究领域：Plant-Microbe Interactions and Immunity、Legume Nitrogen Fixing Symbiosis、Bacteriophages and microbial interactions

The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.