Cloning of an Interleukin-3 Receptor Gene: a Member of a Distinct Receptor Gene Family

作者：Naoto Itoh, Shin Yonehara, Jolanda Schreurs, Daniel M. Gorman, Kazuo Maruyama, Ai Ishii, Ichiro Yahara, Ken‐ichi Arai, Atsushi Miyajima · 发表于：Science · 年份：1990 · DOI：10.1126/science.2404337 · 被引用次数：415 · 研究领域：Immune Cell Function and Interaction、Viral Infectious Diseases and Gene Expression in Insects、Toxin Mechanisms and Immunotoxins

Interleukin-3 (IL-3) binds to its receptor with high and low affinities, induces tyrosine phosphorylation, and promotes the proliferation and differentiation of hematopoietic cells. A binding component of the IL-3 receptor was cloned. Fibroblasts transfected with the complementary DNA bound IL-3 with a low affinity [dissociation constant (Kd) of 17.9 +/- 3.6 nM]. No consensus sequence for a tyrosine kinase was present in the cytoplasmic domain. Thus, additional components are required for a functional high affinity IL-3 receptor. A sequence comparison of the IL-3 receptor with other cytokine receptors (erythropoietin, IL-4, IL-6, and the beta chain IL-2 receptor) revealed a common motif of a distinct receptor gene family.