Genome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.

作者：Jesse W. Summers, Anne R. O’Connell, Irving Millman · 发表于：Proceedings of the National Academy of Sciences · 年份：1975 · DOI：10.1073/pnas.72.11.4597 · 被引用次数：335 · 研究领域：DNA Repair Mechanisms、Hepatitis B Virus Studies、HIV/AIDS drug development and treatment

DNA extracted from Dane particles has been characterized by gel electrophoresis and restriction enzyme cleavage with endonuclease R-HaeIII (from Hemophilus aegyptius). Dane particle DNA is proposed to be a double-stranded circular DNA approximately 3600 nucleotides in length containing a single-stranded gap of 600-2100 nucleotides. The endogenous DNA polymerase (DNA nucleotidyl-transferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase; EC 2.7.7.7) reaction appears to repair this single-stranded gap.