An Amperometric Biosensor Based on the Coimmobilization of Horseradish Peroxidase and Methylene Blue on a Carbon Nanotubes Modified Electrode

作者：Jin‐Zhong Xu, J ZHU, Qiang Wu, Zheng Shui Hu, Hong‐Yuan Chen · 发表于：Electroanalysis · 年份：2003 · DOI：10.1002/elan.200390027 · 被引用次数：208 · 研究领域：Electrochemical sensors and biosensors、Electrochemical Analysis and Applications、Analytical Chemistry and Sensors

Abstract A novel hydrogen peroxide biosensor has been constructed based on the characteristics of the carbon nanotube. The multiwall carbon nanotube (MWNT) was used as a coimmobilization matrix to incorporate horseradish peroxidase (HRP) and electron transfer mediator methylene blue (MB) onto a glassy carbon electrode surface. Cyclic voltammetry and amperometric measurements were employed to demonstrate the feasibility of methylene blue as an electron carrier between the immobilized peroxidase and the surface of glassy carbon electrode. The amperometric response of this resulting biosensor to H 2 O 2 shows a linear relation in the range from 4 μM to 2 mM. The detection limit was 1 μM when the signal to noise ratio is 3. The presence of dopamine and ascorbic acid hardly affects the sensitive determination of H 2 O 2 . This biosensor also possesses very good stability and reproducibility.