B lymphocyte-specific, Cre-mediated mutagenesis in mice

作者：Regan Rickert · 发表于：Nucleic Acids Research · 年份：1997 · DOI：10.1093/nar/25.6.1317 · 被引用次数：738 · 研究领域：RNA Interference and Gene Delivery、Immune Cell Function and Interaction、T-cell and B-cell Immunology

Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.