Мicroscopic investigations (LM, TEM and SEM) and identification ofChlorellaisolate R-06/2 from extreme habitat in Bulgaria with a strong biological activity and resistance to environmental stress factors

作者：Georg Gärtner, Blagoy Uzunov, Elisabeth Ingolić, Werner Kofler, Gergana Gacheva, Plamen S. Pilarski, Lyuben Zagorchev, Mariela Odjakova, Maya Petrova Stoyneva-Gärtner · 发表于：Biotechnology & Biotechnological Equipment · 年份：2015 · DOI：10.1080/13102818.2015.1013283 · 被引用次数：30 · 研究领域：Algal biology and biofuel production、Microbial Community Ecology and Physiology、Protist diversity and phylogeny

An extremophilic Chlorella strain R-06/2, isolated from a geothermal spring (+42 °C) in the region of Rupite village (SW Bulgaria), was investigated for species identification. This was done by observation of the cell morphology, reproduction and ultrastructure by light microscopy, scanning electron microscopy and transmission electron microscopy, and by investigation of the cell-wall chemistry. The pyrenoid ultrastructure with a double-layered thylakoid traversing the matrix, the shape of the starch envelope, as well as the cell wall, composed of glucosamine and developed around young autospores, were the features that allowed us to classify the thermophilic strain Chlorella R-06/2 as Chlorella vulgaris Beijerinck 1890.