Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling

作者：Dietmar Werner Hutmacher, Thorsten Schantz, Iwan Zein, Kee Woei Ng, Swee Hin Teoh, Kim Cheng Tan · 发表于：Journal of Biomedical Materials Research · 年份：2001 · DOI：10.1002/1097-4636(200105)55:2<203::aid-jbm1007>3.0.co;2-7 · 被引用次数：1411 · 研究领域：Bone Tissue Engineering Materials、Additive Manufacturing and 3D Printing Technologies、Electrospun Nanofibers in Biomedical Applications

A number of different processing techniques have been developed to design and fabricate three-dimensional (3D) scaffolds for tissue-engineering applications. The imperfection of the current techniques has encouraged the use of a rapid prototyping technology known as fused deposition modeling (FDM). Our results show that FDM allows the design and fabrication of highly reproducible bioresorbable 3D scaffolds with a fully interconnected pore network. The mechanical properties and in vitro biocompatibility of polycaprolactone scaffolds with a porosity of 61 +/- 1% and two matrix architectures were studied. The honeycomb-like pores had a size falling within the range of 360 x 430 x 620 microm. The scaffolds with a 0/60/120 degrees lay-down pattern had a compressive stiffness and a 1% offset yield strength in air of 41.9 +/- 3.5 and 3.1 +/- 0.1 MPa, respectively, and a compressive stiffness and a 1% offset yield strength in simulated physiological conditions (a saline solution at 37 degrees C) of 29.4 +/- 4.0 and 2.3 +/- 0.2 MPa, respectively. In comparison, the scaffolds with a 0/72/144/36/108 degrees lay-down pattern had a compressive stiffness and a 1% offset yield strength in air of 20.2 +/- 1.7 and 2.4 +/- 0.1 MPa, respectively, and a compressive stiffness and a 1% offset yield strength in simulated physiological conditions (a saline solution at 37 degrees C) of 21.5 +/- 2.9 and 2.0 +/- 0.2 MPa, respectively. Statistical analysis confirmed that the five-angle scaffolds had sig...