Surface immunoglobulins of lipopolysaccharide‐stimulated spleen cells The behaviour of IgM, IgD and IgG

作者：Alain Bourgois, Kumiko Kitajima, I.R. Hunter, Brigitte Alice Askonas · 发表于：European Journal of Immunology · 年份：1977 · DOI：10.1002/eji.1830070307 · 被引用次数：93 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Escherichia coli research studies、T-cell and B-cell Immunology

The nature of Ig receptors carried by lipopolysaccharide (LPS)-stimulated mouse spleen B cells was analyzed by surface iodination, direct antiserum precipitation and polyacrylamide gel electrophoresis. LPS activation led to a rapid decrease of surface IgD to 20 % and 80 % of the original level on days three and five of culture, respectively. Efficiency of iodination of cells doubled after culture but the proportional radioactivity in IgM reace of cells, but only in small amounts (1 % of total surface Ig) and this expression of IgG remained constant during 5 days of culture. We could definitively identify gamma-chains on the surface of cells, but only in small amounts (1% of total surface Ig) and this expression of IgG remained constant during 5 days of culture.