Characterization of a Cell-Lethal Product from the Photooxidation of Tryptophan: Hydrogen Peroxide

作者：J. P. MCCORMICK, John R. Fischer, J. P. PACHLATKO, A. Eisenstark · 发表于：Science · 年份：1976 · DOI：10.1126/science.1108203 · 被引用次数：186 · 研究领域：Protein Interaction Studies and Fluorescence Analysis、Analytical Chemistry and Chromatography、Mass Spectrometry Techniques and Applications

Near-ultraviolet (300 to 400 nanometers) irradiation of saturated, oxygenated solutions of tryptophan in the absence of added sensitizer gives rise to substances that have various biological effects on isolated cells, including mutagenicity and selective lethality to recombination-deficient bacterial mutants. One of these biologically active products has been identified as H2O2, on the basis of spectrometric, chromatographic, chemical, and biological properties. Now H2O2 has been shown to account for the biological activities mentioned above.