Chromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence

作者：Robert T. Simpson, Peter Künzler · 发表于：Nucleic Acids Research · 年份：1979 · DOI：10.1093/nar/6.4.1387 · 被引用次数：208 · 研究领域：DNA and Nucleic Acid Chemistry、Genomics and Chromatin Dynamics、RNA Interference and Gene Delivery

Chicken erythrocyte inner histones (H2A, H2B, H3 and H4) were associated with the two complementary homopolymeric polydeoxyribonucleotides and the two alternating copolymeric polydeoxyribonucleotides. No evidence for formation of chromatin-like structures was obtained for the complexes with poly(dG) . poly(dC) or poly(dA) . poly(dT). Both poly (dGdC) . poly(dGdC) and poly(dAdT) . poly(dAdT) could be folded by histones to yield material digested by DNAase I to multiples of about 10 and by staphylococcal nuclease to 146 bp core particles. Due to the lack of sequence heterogeniety in the complex of histones with poly(dAdT) . poly(dAdT), core particles with remarkable fine structural detail are obtained. The internal organization of DNA in the AT-containing and GC-containing core particles appears not to be identical.