AUTORADIOGRAPHIC STUDIES OF NUCLEIC ACIDS AND PROTEINS DURING MEIOSIS IN LILIUM LONGIFLORUM

作者：James Herbert Taylor · 发表于：American Journal of Botany · 年份：1959 · DOI：10.1002/j.1537-2197.1959.tb07039.x · 被引用次数：88 · 研究领域：Flowering Plant Growth and Cultivation、Ecology and Conservation Studies

Taylor, J. Herbert (Columbia U., New York, N. Y.) Autoradiographic studies of nucleic acids and proteins during meiosis in Lilium longiflorum. Amer. Jour. Bot. 46(7): 477–484. Illus. 1959.—A study was made of the incorporation of glycine‐C14, orotic acid‐C14 and cytidine‐H3 into nucleic acids and proteins of sporogenous and tapetal cells of lily anthers preceding and during meiosis. Methods for differential extraction of nucleic acids from tissue sections, which had been frozen, dehydrated by alcohol‐substitution, and fixed in hot alcohol, were tested by chromatographic analysis of extracts. Both acid and enzyme hydrolysis were shown to be useful for quantitative or, at least, semi‐quantitative work. DNA synthesis was shown to occur only during premeiotic interphase in sporogenous cells, but at two intervals in tapetal nuclei, once when the microsporocytes are in zygotene and again during pachytene. Each time the synthetic period was followed by a normal mitosis. Accumulation of RNA in microsporocytes occurred at stages up to late leptotene. After this period, labeled RNA accumulated almost exclusively in their nuclei and at a slower rate than in earlier stages. DNA synthesis, as measured by incorporation of glycine‐C14 and orotic acid‐C14, gave the same results and confirm earlier results with inorganic phosphate‐P32. For RNA, glycine‐C14 and orotic acid‐C14 gave different results. When glycine‐C14 was the source of label, incorporation of C14 in RNA stopped during DNA synth...