COMBINED BIOCHEMICAL AND MORPHOLOGICAL STUDY OF PARTICULATE FRACTIONS FROM RAT LIVER

作者：Pierre Baudhuin, Henri Beaufay, Christian de Duve · 发表于：The Journal of Cell Biology · 年份：1965 · DOI：10.1083/jcb.26.1.219 · 被引用次数：264 · 研究领域：Gout, Hyperuricemia, Uric Acid、Ion channel regulation and function、Neonatal Health and Biochemistry

Six particulate preparations isolated from rat liver under different experimental conditions were analyzed biochemically and examined in the electron microscope. The results confirm the lysosomal nature of the pericanalicular dense bodies and demonstrate that the microbodies are the bearers of urate oxidase, catalase, and D-amino acid oxidase. Catalase, representing a major component of the particles, and D-amino acid oxidase appear to be associated with the structureless "sap" of the particles, urate oxidase with their crystalloid core or with their outer membrane.