AT_CHLORO, a Comprehensive Chloroplast Proteome Database with Subplastidial Localization and Curated Information on Envelope Proteins

作者：Myriam Ferro, Sabine Brugière, Daniel Salvi, Daphné Seigneurin‐Berny, Magali Court, Lucas Moyet, Claire Ramus, Stéphane Miras, Mourad Mellal, Sophie Le Gall, Sylvie Kieffer‐Jaquinod, Christophe Bruley, Jérôme Garin, Jacques Joyard, Christophe Masselon, Norbert Rolland · 发表于：Molecular & Cellular Proteomics · 年份：2010 · DOI：10.1074/mcp.m900325-mcp200 · 被引用次数：478 · 研究领域：Genomics and Phylogenetic Studies、Photosynthetic Processes and Mechanisms、Advanced Proteomics Techniques and Applications

Recent advances in the proteomics field have allowed a series of high throughput experiments to be conducted on chloroplast samples, and the data are available in several public databases. However, the accurate localization of many chloroplast proteins often remains hypothetical. This is especially true for envelope proteins. We went a step further into the knowledge of the chloroplast proteome by focusing, in the same set of experiments, on the localization of proteins in the stroma, the thylakoids, and envelope membranes. LC-MS/MS-based analyses first allowed building the AT_CHLORO database (http://www.grenoble.prabi.fr/protehome/grenoble-plant-proteomics/), a comprehensive repertoire of the 1323 proteins, identified by 10,654 unique peptide sequences, present in highly purified chloroplasts and their subfractions prepared from Arabidopsis thaliana leaves. This database also provides extensive proteomics information (peptide sequences and molecular weight, chromatographic retention times, MS/MS spectra, and spectral count) for a unique chloroplast protein accurate mass and time tag database gathering identified peptides with their respective and precise analytical coordinates, molecular weight, and retention time. We assessed the partitioning of each protein in the three chloroplast compartments by using a semiquantitative proteomics approach (spectral count). These data together with an in-depth investigation of the literature were compiled to provide accurate subplastidia...