A Study of Passive Immunization Against a Transplanted G+ Leukemia with Specific Antiserum.

作者：Lloyd J. Old, Elisabeth Stockert, Edward A. Boyse, Gayla Geering · 发表于：Experimental Biology and Medicine · 年份：1967 · DOI：10.3181/00379727-124-31667 · 被引用次数：53 · 研究领域：Virus-based gene therapy research、Hepatitis B Virus Studies、T-cell and Retrovirus Studies

Antiserum with high titers of cytotoxic antibody against G-f leukemia cells can be prepared in inbred rats by immunization with histocompatible rat leukemias induced in the same inbred strain by wild-type Gross virus. Under these conditions heteroantibody and isoantibody to normal tissue antigens are not formed. Passive immunization with this antiserum was successful against a G + transplanted mouse leukemia but not against a G— mouse leukemia of the same inbred strain. These results conform to the specificity of the antiserum for G+ cells, as shown by the cytotoxic test in vitro. Complete protection can be achieved in some mice by serum given as late as 3 days after intravenous inoculation of leukemia cells. Protected mice are not immune to rechallenge with minimal numbers of G + leukemia cells after the transferred G antibody has been cleared.