Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways

作者：Yi Wei Jiang, Philippe Veschambre, Hediye Erdjument‐Bromage, Paul Tempst, Joan Weliky Conaway, Ronald C. Conaway, Roger D. Kornberg · 发表于：Proceedings of the National Academy of Sciences · 年份：1998 · DOI：10.1073/pnas.95.15.8538 · 被引用次数：310 · 研究领域：Viral Infectious Diseases and Gene Expression in Insects、CRISPR and Genetic Engineering、Genomics and Chromatin Dynamics

A multiprotein complex isolated from murine cells is identified as a counterpart of the yeast Mediator of transcriptional regulation on the basis of the following: homologs of two subunits of yeast Mediator, Srb7 and Med7, copurify with the complex; peptide sequencing reveals, in addition, homologs of the yeast Mediator subunits Rgr1 and Med6; as with yeast Mediator, the mouse complex binds to the RNA polymerase II C-terminal domain (CTD) and stimulates phosphorylation of the CTD by TFIIH. Peptide sequencing also identifies a component of mouse Mediator as a relative of Ring-3 protein, a mitogen-activated nuclear protein kinase, raising the possibility of Mediator as an end point of signal transduction pathways.