Yeast RNA Polymerase II Genes: Isolation with Antibody Probes

作者：Richard A. Young, Ronald W. Davis · 发表于：Science · 年份：1983 · DOI：10.1126/science.6356359 · 被引用次数：1077 · 研究领域：Fungal and yeast genetics research、RNA and protein synthesis mechanisms、Monoclonal and Polyclonal Antibodies Research

Genes encoding yeast RNA polymerase II subunits were cloned. Efficient isolation of these genes was accomplished by probing a phage lambda gt11 recombinant DNA expression library with polyvalent antibodies directed against purified yeast RNA polymerase II. The identity of genes that specify the largest RNA polymerase II subunits, the 220,000- and 150,000-dalton polypeptides, was confirmed by competitive radioimmune assay. Both of these genes exist in single copy in the yeast Saccharomyces cerevisiae.