A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan

作者：Yoshihiro Kubo, Kenji Takeuchi, Sumalee Boonmar, Tohru Katayama, Qui-Lim Choo, George C. Kuo, Amy J. Weiner, Daniel W. Bradley, Michael Houghton, Izumu Saito, Tatsuo Miyamura · 发表于：Nucleic Acids Research · 年份：1989 · DOI：10.1093/nar/17.24.10367 · 被引用次数：164 · 研究领域：Hepatitis C virus research、Hepatitis B Virus Studies、Animal Virus Infections Studies

Recently, a cDNA from the hepatitis C virus (HCV) RNA genome has been isolated in the USA from a chronically infected chimpanzee. In order to isolate HCV cDNA derived from human material, RNA was extracted from plasma of a Japanese blood donor implicated in post-transfusion non-A, non-B hepatitis and HCV cDNA was synthesized and amplified by the PCR method using HCV-specific oligonucleotide primers. The cDNA fragment, 583 nucleotides long, showed 79.8% homology at the nucleotide level and 92.2% homology at the amino acid level compared with the prototype HCV cDNA. These results provides further evidence to show that HCV is closely associated with the development of post transfusion non-A, non-B hepatitis.