Superoxide Dismutase Isozymes in Different Human Tissues, Their Genetic Control and Intracellular Localization

作者：Gunhild Beckman, Erik Lundgren, Arne Tärnvik · 发表于：Human Heredity · 年份：1973 · DOI：10.1159/000152594 · 被引用次数：75 · 研究领域：Mitochondrial Function and Pathology、Glutathione Transferases and Polymorphisms、Metal-Catalyzed Oxygenation Mechanisms

Two isozymes of superoxide dismutase, A and B, were found in extracts of human organs, tissues and cell cultures. Differential centrifugation of cell homogenates revealed that isozyme A was localized in the soluble phase and isozyme B in fractions enriched in mitochondria. Isozyme B was lacking in erythrocytes and isozyme A in polymorphonuclear leucocytes. All other specimens showed both isozymes although in variable proportions. Evidence was presented suggesting that isozymes A and B are controlled by genes at separate gene loci. The occurrence of hybrid enzyme in a heterozygous phenotype of isozyme A suggests that the isozyme is a dimer made up of two identical polypeptide subunits.