Induction of nitric oxide synthase in demyelinating regions of multiple sclerosis brains

作者：Lars Eirik Bø, Ted M. Dawson, Steve L Wesselingh, Sverre Möurk, Steven J. Choi, Pei Ann Kong, Daniel F. Hanley, Bruce D. Trapp · 发表于：Annals of Neurology · 年份：1994 · DOI：10.1002/ana.410360515 · 被引用次数：572 · 研究领域：Electron Spin Resonance Studies、Nitric Oxide and Endothelin Effects、Redox biology and oxidative stress

The amount of messenger RNA encoding human inducible nitric oxide synthase and the presence and distribution of NADPH diaphorase were determined in tissue sections from multiple sclerosis (MS) and control brains. Levels of human nitric oxide synthase messenger RNA were markedly elevated in MS brains when compared to normal control brains. NADPH diaphorase activity, a histochemical stain reflecting nitric oxide synthase catalytic activity, was detected in reactive astrocytes in active demyelinating MS lesions and at the edge of chronic active demyelinating lesions. Control brains did not contain NADPH diaphorase-positive astrocytes. These results implicate the free radical nitric oxide in the pathogenesis of demyelinating MS lesions.