A cost-effective RNA sequencing protocol for large-scale gene expression studies

作者：Zhonggang Hou, Peng Jiang, Scott Swanson, Angela L. Elwell, Bao Kim Nguyen, Jennifer M. Bolin, Ron Stewart, James A. Thomson · 发表于：Scientific Reports · 年份：2015 · DOI：10.1038/srep09570 · 被引用次数：103 · 研究领域：Genomics and Phylogenetic Studies、Single-cell and spatial transcriptomics、RNA modifications and cancer

RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels.