A Technique for Expressing Eukaryotic Genes in Bacteria

作者：Leonard P. Guarente, Thomas M. Roberts, Mark Ptashne · 发表于：Science · 年份：1980 · DOI：10.1126/science.6158095 · 被引用次数：38 · 研究领域：Bacterial Genetics and Biotechnology、RNA and protein synthesis mechanisms、Bacteriophages and microbial interactions

Methods are described that allow efficient expression in Escherichia coli of cloned eukaryotic genes. The methods require that the coding sequence of the gene in question be available in a form uninterrupted by intervening sequences (for example, as a complementary DNA clone). The gene products are synthesized unfused to other amino acid sequences. The genetic manipulations are simple, and require the plasmids described and commercially available enzymes.