UROPEPSIN EXCRETION BY MAN. I. THE SOURCE, PROPERTIES AND ASSAY OF UROPEPSIN 12

作者：I. Arthur Mirsky, S. Block, Stanley Osher, Robert H. Broh-Kahn · 发表于：Journal of Clinical Investigation · 年份：1948 · DOI：10.1172/jci102034 · 被引用次数：87 · 研究领域：Protein Hydrolysis and Bioactive Peptides、Bee Products Chemical Analysis、Antimicrobial Peptides and Activities

The conventional method used to study gastric secretion and activity involves the withdrawal and examination of specimens of gastric juice. Such a procedure subjects the average patient to con-siderable physical and emotional stresses. Fur-ther, the repeated collection of samples of gastric juice from a great number of individuals over a prolonged period of time presents many practical difficulties. It would, therefore, be advantageous to possess a means for the study of gastric activity which would have none of the disadvantages in-volved in the withdrawal of the gastric contents. During the course of studies designed to develop some technique for this purpose, we investigated the excretion of uropepsin, a pepsin-like enzyme in the urine. The presence of an enzyme in the urine, with proteolytic activity at strongly acid reactions, was first demonstrated by Brucke (1). Since that time occasional investigators have reported informa-tion concerning the nature of this substance, its source, and the factors controlling its appearance in the urine. Unfortunately much of the early work in this field was accomplished with the aid of methods of a questionable degree of accuracy. In particular this is true for the procedures utilized for the determination of the activity of this sub-stance. Accordingly, much of this work, which has been summarized in an excellent review by Bucher (2), requires confirmation. More recent studies by Farnsworth, Speer and Alt (3) and by Bucher (2), have r...