Rate of Elongation of Polyphenylalanine in vitro

作者：E. Gerhart H. Wagner, Pierre C. Jelenc, Maans Ehrenberg, C. G. Kurland · 发表于：European Journal of Biochemistry · 年份：1982 · DOI：10.1111/j.1432-1033.1982.tb05866.x · 被引用次数：110 · 研究领域：RNA and protein synthesis mechanisms、Biopolymer Synthesis and Applications、Antimicrobial Peptides and Activities

Ribosomes purified from Escherichia coli were preincubated with AcPhe-tRNA and poly(U). Then purified components necessary for polypeptide synthesis were added. Incubation of the complete system led to a burst of elongation which lasted for nearly 10 s. During the initial burst approximately 10% of the ribosomes participated in the elongation of poly(Phe) at an average rate per ribosome close to eight peptide bonds/s. The missense error rate with leucine was 4 x 10(-4) during the burst. Accordingly, the preincubated elongation system functions at a rate, as well as an accuracy, close to those of protein synthesis in vivo.