Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile

作者：Ann E. Cowan, Elizabeth M. Olivastro, Dennis E. Koppel, Charles A. Loshon, Barbara Setlow, Peter Setlow · 发表于：Proceedings of the National Academy of Sciences · 年份：2004 · DOI：10.1073/pnas.0306859101 · 被引用次数：213 · 研究领域：Bacterial Genetics and Biotechnology、Lipid Membrane Structure and Behavior、Amino Acid Enzymes and Metabolism

Bacterial spores of various Bacillus species are impermeable or exhibit low permeability to many compounds that readily penetrate germinated spores, including methylamine. We now show that a lipid probe in the inner membrane of dormant spores of Bacillus megaterium and Bacillus subtilis is largely immobile, as measured by fluorescence redistribution after photobleaching, but becomes free to diffuse laterally upon spore germination. The lipid immobility in and the slow permeation of methylamine through the inner membrane of dormant spores may be due to a significant (1.3- to 1.6-fold) apparent reduction of the membrane surface area in the dormant spore relative to that in the germinated spore, but is not due to the dormant spore's high levels of dipicolinic acid and divalent cations.