Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity

作者：Rute Madeira Lau, Menno J. Sorgedrager, Giacomo Carrea, Fred van Rantwijk, Francesco Secundo, Roger A. Sheldon · 发表于：Green Chemistry · 年份：2004 · DOI：10.1039/b405693k · 被引用次数：318 · 研究领域：Ionic liquids properties and applications、Enzyme Catalysis and Immobilization、Electrochemical sensors and biosensors

The effects of ionic liquid media on the activity of Candida antarctica lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF4]. Only [Et3MeN][MeSO4] was an exception, as dissolved CaLB maintained its activity in this solvent. By means of FT-IR spectroscopy, denaturation of CaLB was observed upon dissolution in ionic liquids in which the activity was low, whereas the conformation of enzyme dissolved in [Et3MeN][MeSO4] closely resembled the native one.