LYSIS FROM WITHOUT OF S. AUREUS K1 BY THE COMBINED ACTION OF PHAGE AND VIROLYSIN

作者：Doris J. Ralston, B. S. Baer, Miriam R. Lieberman, ALBERT PAUL KRUEGER · 发表于：The Journal of General Physiology · 年份：1957 · DOI：10.1085/jgp.41.2.343 · 被引用次数：26 · 研究领域：Bacteriophages and microbial interactions、Microbial infections and disease research、Bacillus and Francisella bacterial research

LYSIS FROM WITHOUT (LFW) OCCURS IN TWO STEPS: (1) sensitization of cells by phage, which renders the cells susceptible to (2) destruction of an essential cell structure by an extracellular lytic enzyme. Virolysin, from phage-infected cells, was used in these studies. Normal cell autolysin is also effective. Evidence is presented that: 1. Neither phage nor lysin alone causes LFW. 2. Sensitization requires phage adsorption. 3. It can be caused by non-infectious particles. This establishes a new biological activity of the particle. 4. Heat, U.V., detergents, penicillin, and other damaging agents also sensitize cells. 5. Sensitization involves a non-lethal, reversible reaction. 6. Sensitization by phage prevents virus synthesis. Following adsorption, a cell can undergo sensitization or infection but not simultaneously. When only a few particles are adsorbed, infection can occur; when sufficient particles are adsorbed, sensitization takes place. 7. Quantitative aspects of LFW are described. Lysis proceeds logarithmically. The lysis end-point depends upon the phage concentration but is independent of the enzyme concentration.