Silver Nanoparticles Crossing Through and Distribution in the Blood-Brain Barrier In Vitro

作者：Jinglong Tang, Ling Xiong, Guofeng Zhou, Shuo Wang, Jianyu Wang, Li Liu, Jiage Li, Fuqiang Yuan, Songfang Lu, Ziyi Wan, Laisheng Lee Chou, Tingfei Xi · 发表于：Journal of Nanoscience and Nanotechnology · 年份：2010 · DOI：10.1166/jnn.2010.2625 · 被引用次数：142 · 研究领域：Nanoparticles: synthesis and applications、Barrier Structure and Function Studies、Gold and Silver Nanoparticles Synthesis and Applications

Silver nanoparticles (SNPs) translocate to the brain through the blood stream after they are implanted in vivo. The aim of this study was to investigate the distribution of SNPs that crossed through the blood-brain barrier (BBB). An in vitro BBB model established by co-cultures of rat brain microvessel vascular endothelial cells (BMVECs) with astrocytes (ACs) was cultured with cell culture medium containing 100 microg/mL of either SNPs or silver microparticles (SMPs). After 4 hours of culture, the ultrastructure and its silver content of BBB was evaluated with transmission electronic microscopy (TEM) and inductively-coupled plasma mass spectrometry (ICP-MS) respectively. Results demonstrated that SNPs crossed the BBB and accumulated inside BMVECs, while the SMPs did not. The data indicated a special distribution of SNPs in the BBB and suggested that SNPs pass the BBB mainly by transcytosis of capillary endothelial cells. Further study would be necessary to evaluate the actual biological effects of SNPs on the brain.