Wild-type p53 binds to the TATA-binding protein and represses transcription.

作者：Edward Seto, Anny Usheva, Gerard P. Zambetti, Jamil Antonio Momand, Nobuo Horikoshi, Roberto Simon Weinmann, Arnold J. Levine, Thomas E. Shenk · 发表于：Proceedings of the National Academy of Sciences · 年份：1992 · DOI：10.1073/pnas.89.24.12028 · 被引用次数：522 · 研究领域：RNA Research and Splicing、Cancer-related Molecular Pathways、RNA modifications and cancer

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.