Environmental Stimuli in the Progression of BHK/21 Cells Through the Cell Cycle

作者：M. Shodell · 发表于：Proceedings of the National Academy of Sciences · 年份：1972 · DOI：10.1073/pnas.69.6.1455 · 被引用次数：41 · 研究领域：Pancreatic function and diabetes、Viral Infectious Diseases and Gene Expression in Insects、T-cell and B-cell Immunology

An L-cell line adapted to grow in protein-free medium is a source of material that stimulates the proliferation of serum-dependent BHK cells under otherwise protein-free conditions. The material produced by the L-cells is resolvable into separate DNA-synthetic and mitotic stimulatory activities. The stimulation of mitosis is dependent upon the presence of one or more heat-labile macromolecules, which can be concentrated by ultrafiltration, and at least one dialyzable component is essential for the initiation of DNA synthesis.