In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

作者：Ramaswamy Narayanan, Margaret M. Jastreboff, C.-F. Chiu, Joseph R. Bertino · 发表于：Biochemical and Biophysical Research Communications · 年份：1986 · DOI：10.1016/s0006-291x(86)80146-2 · 被引用次数：25 · 研究领域：Microbial Inactivation Methods、Viral Infectious Diseases and Gene Expression in Insects、Transgenic Plants and Applications

Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.