A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo.

作者：Atsushi Suzuki, R. Scott Thies, Noboru Yamaji, Jeffrey J. Song, John M. Wozney, Kouichi MURAKAMI, Naoto Ueno · 发表于：Proceedings of the National Academy of Sciences · 年份：1994 · DOI：10.1073/pnas.91.22.10255 · 被引用次数：456 · 研究领域：Developmental Biology and Gene Regulation、TGF-β signaling in diseases、Hedgehog Signaling Pathway Studies

Bone morphogenetic proteins (BMPs), which are members of the transforming growth factor beta (TGF-beta) superfamily, have been implicated in bone formation and the regulation of early development. To better understand the roles of BMPs in Xenopus laevis embryogenesis, we have cloned a cDNA coding for a serine/threonine kinase receptor that binds BMP-2 and BMP-4. To analyze its function, we attempted to block the BMP signaling pathway in Xenopus embryos by using a dominant-negative mutant of the BMP receptor. When the mutant receptor lacking the putative serine/threonine kinase domain was expressed in ventral blastomeres of Xenopus embryos, these blastomeres were respecified to dorsal mesoderm, eventually resulting in the formation of a secondary body axis. These findings suggest that endogenous BMP-2 and BMP-4 are involved in the dorsal-ventral specification in the embryo and that ventral fate requires induction rather than resulting from an absence of dorsal specification.