Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside.

作者：Barbara Ranscht, Patric A. Clapshaw, Jack Price, Mark Noble, Wilfried E. Seifert · 发表于：Proceedings of the National Academy of Sciences · 年份：1982 · DOI：10.1073/pnas.79.8.2709 · 被引用次数：836 · 研究领域：Neurogenesis and neuroplasticity mechanisms、Glycosylation and Glycoproteins Research、Cellular transport and secretion

We have generated a hybridoma cell line secreting a monoclonal antibody that specifically binds to the surfaces of oligodendrocytes and Schwann cells, the cells involved in myelin formation in the central and peripheral nervous systems, respectively. Binding studies using purified sphingolipids showed that this antibody reacts strongly with galactocerebroside (GalC), the major galactosphingolipid of myelin. The antibody was used in conjunction with rabbit antisera against sulfatide, the sulfated form of GalC, to examine the developmental appearance of these lipids on the surfaces of oligodendrocytes and Schwann cells. In addition, the loss of GalC and sulfatide from freshly dissociated Schwann cells was compared. These studied showed that GalC is expressed on the cell surface prior to sulfatide on both of these cell types in vivo and in vitro. Conversely, dissociated Schwann cells lose their cell surface sulfatide more rapidly than they lose their surface GalC under nonmyelinating conditions.