Empirical Bayes Analysis of a Microarray Experiment

作者：Bradley Efron, Robert John Tibshirani, John D. Storey, Virginia Goss Tusher · 发表于：Journal of the American Statistical Association · 年份：2001 · DOI：10.1198/016214501753382129 · 被引用次数：1793 · 研究领域：Gene expression and cancer classification、Bioinformatics and Genomic Networks、Molecular Biology Techniques and Applications

Microarrays are a novel technology that facilitates the simultaneous measurement of thousands of gene expression levels. A typical microarray experiment can produce millions of data points, raising serious problems of data reduction, and simultaneous inference. We consider one such experiment in which oligonucleotide arrays were employed to assess the genetic effects of ionizing radiation on seven thousand human genes. A simple nonparametric empirical Bayes model is introduced, which is used to guide the efficient reduction of the data to a single summary statistic per gene, and also to make simultaneous inferences concerning which genes were affected by the radiation. Although our focus is on one specific experiment, the proposed methods can be applied quite generally. The empirical Bayes inferences are closely related to the frequentist false discovery rate (FDR) criterion.