Three-dimensional imaging of biological cells with picosecond ultrasonics

作者：Sorasak Danworaphong, Motonobu Tomoda, Yuki Matsumoto, Osamu Matsuda, Toshiro OHASHI, Hiromu Watanabe, Masafumi Nagayama, Kazutoshi Gohara, Paul H. Otsuka, Oliver B. Wright · 发表于：Applied Physics Letters · 年份：2015 · DOI：10.1063/1.4918275 · 被引用次数：73 · 研究领域：Advanced Fluorescence Microscopy Techniques、Spectroscopy Techniques in Biomedical and Chemical Research、Microfluidic and Bio-sensing Technologies

We use picosecond ultrasonics to image animal cells in vitro—a bovine aortic endothelial cell and a mouse adipose cell—fixed to Ti-coated sapphire. Tightly focused ultrashort laser pulses generate and detect GHz acoustic pulses, allowing three-dimensional imaging (x, y, and t) of the ultrasonic propagation in the cells with ∼1 μm lateral and ∼150 nm depth resolutions. Time-frequency representations of the continuous-wavelet-transform amplitude of the optical reflectivity variations inside and outside the cells show GHz Brillouin oscillations, allowing the average sound velocities of the cells and their ultrasonic attenuation to be obtained as well as the average bulk moduli.