Osteochondral Repair Using Perichondrial Cells: A 1-Year Study in Rabbits

作者：Constance R. Chu, Jon S. Dounchis, Makoto Yoshioka, Robert L. Sah, Richard D. Coutts, David Amiel · 发表于：Clinical Orthopaedics and Related Research · 年份：1997 · DOI：10.1097/00003086-199707000-00029 · 被引用次数：148 · 研究领域：Osteoarthritis Treatment and Mechanisms、Knee injuries and reconstruction techniques、Lower Extremity Biomechanics and Pathologies

Articular cartilage repair remains a clinical and scientific challenge with increasing interest focused on the transplantation of chondrogenic cells. This study evaluated the repair response during a 1-year period after implantation of allogenic perichondrium cell polylactic acid composite grafts into 3.7 x 5 mm osteochondral defects drilled into the medial femoral condyles of 82 adult New Zealand White rabbits. The repair tissue was evaluated grossly, histologically, histomorphometrically, biochemically, and biomechanically at 6 weeks, 12 weeks, 6 months, and 1 year after implantation. After gross evaluation, cartilaginous material appeared to fill the defect in 70 experimental knees, for an overall repair frequency of 85%. The histomorphometric results and the histologic appearances were variable. None of the specimens were completely normal at 1 year. Only specimens with subchondral bone reformation displayed a definable cartilage appearing surface with chondrocytes surrounded by dense matrix. Subchondral bone reformation was inconsistent, reaching 50% at 1 year. Biochemically, the repair tissue matured during a 1-year period into a hyaline Type II collagen dominant tissue, whereas glycosaminoglycan content remained low at all time periods. The measured compressive properties of the repair tissue at 1 year were not significantly different from those of the contralateral knee that was not surgically treated. The treatment of osteochondral defects in the rabbit knee with all...