Direct Electrochemistry of Glucose Oxidase and Biosensing for Glucose Based on Graphene

作者：Changsheng Shan, Huafeng Yang, Jiangfeng Song, Dongxue Han, Ari Ivaska, Li Niu · 发表于：Analytical Chemistry · 年份：2009 · DOI：10.1021/ac802193c · 被引用次数：1314 · 研究领域：Electrochemical sensors and biosensors、Conducting polymers and applications、Advanced biosensing and bioanalysis techniques

We first reported that polyvinylpyrrolidone-protected graphene was dispersed well in water and had good electrochemical reduction toward O(2) and H(2)O(2). With glucose oxidase (GOD) as an enzyme model, we constructed a novel polyvinylpyrrolidone-protected graphene/polyethylenimine-functionalized ionic liquid/GOD electrochemical biosensor, which achieved the direct electron transfer of GOD, maintained its bioactivity and showed potential application for the fabrication of novel glucose biosensors with linear glucose response up to 14 mM.