An improved separation procedure for nucleoside monophosphates on polyethyleneimine- (PEI-)cellulose thin layers

作者：Ramesh Chand Gupta, Erika Randerath, Kurt Randerath · 发表于：Nucleic Acids Research · 年份：1976 · DOI：10.1093/nar/3.11.2915 · 被引用次数：31 · 研究领域：RNA modifications and cancer、RNA and protein synthesis mechanisms、RNA Research and Splicing

A procedure is described for the two-dimensional separation of the 4 major and 16 modified nucleoside-(5') monophosphates on anion-exchange thin layers of polyethyleneimine- (PEI-)cellulose. The method, which is simple and less time-consuming than existing partition chromatographic methods, may be used for the identification of 5'-termini of RNA and RNA fragments.