The Isolation of Antibiotic-Resistant Salmonella from Retail Ground Meats

作者：David Gordon White, Shaohua Zhao, Robert Sudler, Sherry L. Ayers, Sharon L. Friedman, Sheng Chen, Patrick F. McDermott, Shawn D. McDermott, David D. Wagner, Jianghong Meng · 发表于：New England Journal of Medicine · 年份：2001 · DOI：10.1056/nejmoa010315 · 被引用次数：554 · 研究领域：Salmonella and Campylobacter epidemiology、Antibiotic Resistance in Bacteria、Vibrio bacteria research studies

BACKGROUND: Salmonella is a leading cause of foodborne illness. The emergence of antimicrobial-resistant salmonella is associated with the use of antibiotics in animals raised for food; resistant bacteria can be transmitted to humans through foods, particularly those of animal origin. We identified and characterized strains of salmonella isolated from ground meats purchased in the Washington, D.C., area. METHODS: Salmonella was isolated from samples of ground chicken, beef, turkey, and pork purchased at three supermarkets. The isolates were characterized by serotyping, antimicrobial-susceptibility testing, phage typing, and pulsed-field gel electrophoresis. The polymerase chain reaction and DNA sequencing were used to identify resistance integrons and extended spectrum beta-lactamase genes. RESULTS: Of 200 meat samples, 41 (20 percent) contained salmonella, with a total of 13 serotypes. Eighty-four percent of the isolates were resistant to at least one antibiotic, and 53 percent were resistant to at least three antibiotics. Sixteen percent of the isolates were resistant to ceftriaxone, the drug of choice for treating salmonellosis in children. Bacteriophage typing identified four isolates of Salmonella enterica serotype typhimurium definitive type 104 (DT104), one of DT104b, and two of DT208. Five isolates of S. enterica serotype agona had resistance to 9 antibiotics, and the two isolates of serotype typhimurium DT208 were resistant to 12 antibiotics. Electrophoretic patterns...