Fluorescence in the tandem scanning microscope

作者：ALAN BOYDE, Sheila J. Jones, M. Louise Taylor, Laurie A Wolfe, Timothy Frederick Watson · 发表于：Journal of Microscopy · 年份：1990 · DOI：10.1111/j.1365-2818.1990.tb02945.x · 被引用次数：36 · 研究领域：Photoacoustic and Ultrasonic Imaging、Dental materials and restorations、Advanced Fluorescence Microscopy Techniques

Our studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real-time viewing and real colour, which allow faster use and interpretation. CSLM are complementary, not competitive, being currently more sophisticated for low-level fluorescence work. This is equally possible with available TSM, but requires further development using CCD cameras and image-processing systems.