A Simplified Method for the Quantitative Determination of Testosterone-Estradiol-Binding Globulin Activity in Human Plasma

作者：William Rosner · 发表于：The Journal of Clinical Endocrinology & Metabolism · 年份：1972 · DOI：10.1210/jcem-34-6-983 · 被引用次数：347 · 研究领域：Hormonal and reproductive studies、Pharmacological Effects and Assays、Pesticide Residue Analysis and Safety

A rapid and simple method for the measurement of testosterone-estradiol-binding globulin in human plasma is described. The bind- ing protein is saturated with dihydrotestosterone (DHT), precipitated with 50% ammonium sul- fate, and the mass of bound DHT determined from the known specific activity of the added steroid. Normal values, expressed as M-g DHT bound/100 ml serum are: men, 0.93 ±. 0.06 (SEM); women, 1.85 ± 0.13; pregnancy, 12.4 ± 0.67. Elevated values were observed in men with cirrhosis of the liver and patients with thyrotoxi- cosis. Normal values were observed in abstinent alcoholics and in pregnant subjects with diabetes or hypertension. (/ Clin Endocrinol Metab 34: 983, 1972)