Three-dimensional structure of recombinant human interferon-gamma

作者：S.E. Ealick, William J. Cook, Senadhi Vijay‐Kumar, Michael A. Carson, T. L. NAGABHUSHAN, Paul P. Trotta, Charles E. Bugg · 发表于：Science · 年份：1991 · DOI：10.1126/science.1902591 · 被引用次数：431 · 研究领域：interferon and immune responses、Cytokine Signaling Pathways and Interactions、Monoclonal and Polyclonal Antibodies Research

The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is primarily alpha helical, with six helices in each subunit that comprise approximately 62 percent of the structure; there is no beta sheet. The dimeric structure of human interferon-gamma is stabilized by the intertwining of helices across the subunit interface with multiple intersubunit interactions.