Laboratory Diagnosis of Clostridium difficile -Associated Diarrhea and Colitis: Usefulness of Premier Cytoclone A+B Enzyme Immunoassay for Combined Detection of Stool Toxins and Toxigenic C. difficile Strains

作者：Alain Lozniewski, Christian Rabaud, Emmanuelle Dotto, Michèle Weber, Francine Mory · 发表于：Journal of Clinical Microbiology · 年份：2001 · DOI：10.1128/jcm.39.5.1996-1998.2001 · 被引用次数：64 · 研究领域：Clostridium difficile and Clostridium perfringens research、Microscopic Colitis、Viral gastroenteritis research and epidemiology

Detection of Clostridium difficile toxins A and B in stools by Premier Cytoclone A+B enzyme immunoassay (EIA) was compared with detection by stool culture for C. difficile followed by detection of toxigenic isolates using the same EIA. Chart reviews were performed to evaluate the likelihood of C. difficile-associated diarrhea and colitis (CADC) for all patients with at least one positive toxin assay. While the toxins were detected in 58 of 85 consecutive CADC patients by both assays, CADC in 5 patients was detected only by stool toxin assay, and in 22 patients CADC was detected only by toxigenic culture. Our results suggest that for laboratories using a rapid toxin A+B EIA, direct toxin detection in stools should be combined with toxigenic culture in cases in which there is a negative stool toxin assay.