Cleavage of double helical DNA by methidium-propyl-EDTA-iron(II)

作者：Robert Hertzberg, Peter B. Dervan · 发表于：Journal of the American Chemical Society · 年份：1982 · DOI：10.1021/ja00365a069 · 被引用次数：410 · 研究领域：DNA and Nucleic Acid Chemistry、Advanced biosensing and bioanalysis techniques

We report the synthesis of a simple bifunctional molecule, methidiumpropyl-EDTA (MPE) (1), which contains the DNA intercalator methidium covalently bound by a short hydrocarbon tether to the metal chelator EDT A. In the presence of ferrous ion and oxygen this reagent efficiently produces single-strand breaks and some double-strand breaks in double helical DNA.