Epidermal expression of apolipoprotein E gene during fin and scale development and fin regeneration in zebrafish

作者：M J Monnot, Patrick J. Babin, Germán A. Poleo, Michèle André, Lynda Laforest, Chantal Ballagny, Marie‐Andrée Akimenko · 发表于：Developmental Dynamics · 年份：1999 · DOI：10.1002/(sici)1097-0177(199903)214:3<207::aid-aja4>3.0.co;2-5 · 被引用次数：77 · 研究领域：Zebrafish Biomedical Research Applications、Cellular transport and secretion、Neurobiology and Insect Physiology Research

Apolipoprotein E (apoE) plays an important role in systemic and local lipid homeostasis. We have examined the expression of apoE during morphogenesis and regeneration of paired and unpaired fins and during scale development in zebrafish (Danio rerio). In situ hybridization analysis revealed that, during embryogenesis, apoE is expressed in the epithelial cells of the median fin fold and of the pectoral fin buds. ApoE remains expressed in the elongating fin folds throughout development of the fins. During the larval to juvenile transition, apoE transcripts were present in the distal, interray and lateral epidermis of developing fins. Furthermore, as scale buds started to form, apoE was expressed in large scale domains which later, became restricted to the external posterior epidermal part of scales. A low level of transcripts could be observed at later developmental stages at these locations probably because fins and scales continue to grow throughout the animal's life. During regeneration of both pectoral and caudal fins, a marked increase in apoE expression is observed as early as 12 hours after amputation in the wound epidermis. High levels of apoE transcripts are then localized primarily in the basal cell layer of the apical epidermis. The levels of apoE expression were maximum between the second to fourth days and then progressively declined to basal level by day 14. ApoE transcripts were also observed in putative macrophages infiltrated in the mesenchymal compartment of r...