Desorption/ionization of biomolecules from aqueous solutions at atmospheric pressure using an infrared laser at 3 μm

作者：Victor V. Laiko, Nelli I. Taranenko, Vadym D. Berkout, Mikhail A. Yakshin, Coorg R. Prasad, H. Sang Lee, Vladimir M. Doroshenko · 发表于：Journal of the American Society for Mass Spectrometry · 年份：2002 · DOI：10.1016/s1044-0305(02)00341-0 · 被引用次数：104 · 研究领域：Mass Spectrometry Techniques and Applications、Analytical Chemistry and Chromatography、Microfluidic and Capillary Electrophoresis Applications

A new atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) ion source was developed and interfaced with a Thermo Finnigan LCQ ion trap mass spectrometer. The source utilized a miniature all-solid-state optical parametric oscillator (OPO)-based IR laser system tunable in the lambda = 1.5-4 microm spectral range and a nitrogen ultraviolet (UV) laser (lambda = 337 nm) for use in comparative studies. The system demonstrated comparable performance at 3 microm and 337 nm wavelengths if UV matrices were used. However, AP IR-MALDI using a 3 microm wavelength showed good performance with a much broader choice of matrices including glycerol and liquid water. AP IR-MALDI mass spectra of peptides in the mass range up to 2000 Da were obtained directly from aqueous solutions at atmospheric conditions for the first time. A potential use of the new AP IR-MALDI ion source includes direct MS analysis of biological cells and tissues in a normal atmospheric environment as well as on-line coupling of mass spectrometers with liquid separation techniques.