Replication and Transcription of Eukaryotic DNA in Esherichia coli

作者：John F. Morrow, Stanley N. Cohen, Annie C. Y. Chang, Herbert W. Boyer, Howard M. Goodman, Robert B. Helling · 发表于：Proceedings of the National Academy of Sciences · 年份：1974 · DOI：10.1073/pnas.71.5.1743 · 被引用次数：383 · 研究领域：Bacterial Genetics and Biotechnology、DNA Repair Mechanisms、CRISPR and Genetic Engineering

Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.