Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

作者：A J Brake, James P. Merryweather, Daniel G. Coit, Ulrike Heberlein, Frank R. Masiarz, Guy T. Mullenbach, M S Urdea, Pablo Valenzuela, P J Barr · 发表于：Proceedings of the National Academy of Sciences · 年份：1984 · DOI：10.1073/pnas.81.15.4642 · 被引用次数：493 · 研究领域：Transgenic Plants and Applications、Viral Infectious Diseases and Gene Expression in Insects、Fungal and yeast genetics research

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.